| Start | End | School | Mascot |
|---|---|---|---|
| 1937 | Augusta | Panthers | |
| 1970 | 1996 | Bath County | Wildcats |
| 1997 | Bishop Brossart | Mustangs | |
| 1937 | Berry | Eagles | |
| 1937 | Bourbon (Millersburg) | Cardinals | |
| 1937 | Brooksville/Bracken County | Polar Bears | |
| 1964 | 1965 | Bryan Station | Defenders |
| 1937 | 1950 | Buena Vista | Cardinals |
| 1939 | 1959 | Butler | Bobcats |
| 2006 | Calvary Christian | Cougars | |
| 1997 | Campbell County | Camels | |
| 1937 | 1963 | Carlisle | Musketeers |
| 1937 | 1948 | Centerhill | Thorobreds |
| 1938 | 1948 | Clintonville | Pirates |
| Connorsville | Bearcats | ||
| Cynthiana | Bulldogs | ||
| Deming/Robertson County | Black Devils | ||
| Estill County | Engineers | ||
| Ewing | Pirates | ||
| Falmouth | Red Devils | ||
| Flemingsburg | Bobcats | ||
| Fleming County | Panthers | ||
| Georgetown | Buffaloes | ||
| Grant County | Braves | ||
| Great Crossing | Apes | ||
| Harrison County | Thorobreds | ||
| Headquarters | Blue Jackets | ||
| Lewisburg | Golden Bears | ||
| Lewis County | Lions | ||
| Little Rock | Tigers | ||
| Mason County | Royals | ||
| Mayslick | Cardinals | ||
| Maysville | Bulldogs | ||
| Millersburg Military | Cadets | ||
| Milford | |||
| Minerva | |||
| Montgomery County | |||
| Morgan | |||
| 1966 | 1976 | Mt. Sterling | |
| Nicholas County | |||
| North Middletown | |||
| Oddville | |||
| Orangeburg | |||
| Oxford | |||
| Paris | |||
| Paris Western | |||
| Pendleton County | |||
| Renaker | |||
| Ruddles Mill | |||
| Saideville | |||
| Scott | |||
| Scott County | |||
| 1997 | 2019 | Silver Grover | |
| Stamping Ground | Stampers | ||
| St. Agatha | |||
| 1939 | St. Patrick | Saints | |
| Sunrise | Panthers | ||
| Tollesboro | Wildcats | ||
| Vanceburg | Lions | ||
| Washington | Pioneers | ||
| Williamstown | Demons |
